Korean J Physiol Pharmacol 2024; 28(5): 457-467

Published online September 1, 2024 https://doi.org/10.4196/kjpp.2024.28.5.457

Copyright © Korean J Physiol Pharmacol.

Construction and validation of a synthetic phage-displayed nanobody library

Minju Kim^1,2,#, Xuelian Bai^3,#, Hyewon Im⁴, Jisoo Yang^1,2, Youngju Kim^1,2, Minjoo MJ Kim^1,2, Yeonji Oh³, Yuna Jeon³, Hayoung Kwon⁵, Seunghyun Lee^3,*, and Chang-Han Lee^{1,2,4,5,6,7,8,*}

¹Department of Biomedical Sciences, ²BK21 FOUR Biomedical Science Project, Seoul National University College of Medicine, Seoul 03080, ³Research Center, EPD Biotherapeutics Inc., Seoul 08378, ⁴Cancer Research Institute, ⁵Department of Pharmacology, Seoul National University College of Medicine, Seoul 03080, ⁶Wide River Institute of Immunology, Seoul National University, Hongcheon 25159, ⁷SNU Dementia Research Center, College of Medicine, Seoul National University, ⁸Seoul National University Hospital, Seoul 03080, Korea

Correspondence to:Seunghyun Lee
E-mail: sh.lee@epdbio.com
Chang-Han Lee
E-mail: chlee-antibody@snu.ac.kr

^#These authors contributed equally to this work.

Author contributions: C.H.L. and S.L. conceptualized the study. C.H.L., S.L., X.B., Y.O., and Y.J. were responsible for the design of nanobody library. M.K., H.I., J.Y., Y.K., and M.M.K. constructed nanobody library. M.K., H.I., X.B., Y.O., and Y.J. carried out the biopanning experiments. M.K. and H.I. preformed the protein purification and ELISA experiments. In silico analysis was conducted by M.K., and C.H.L., S.L., M.K., H.I., and H.K. analyzed the data. C.H.L., S.L., and M.K. wrote the manuscript.