Korean J Physiol Pharmacol 2024; 28(4): 379-387
Published online July 1, 2024 https://doi.org/10.4196/kjpp.2024.28.4.379
Copyright © Korean J Physiol Pharmacol.
Suli Xu1,*, Zhao Ma1, Lihua Xing2, and Weiqing Cheng3
1Department of Medicine, Huangshan Vocational Technical College, Huangshan, Anhui 245000, 2School of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui 230012, 3Department of Pharmacy, Fujian Vocational College of Bioengineering, Fuzhou, Fujian 350007, China
Correspondence to:Suli Xu
E-mail: xusuli198210@163.com
Breast cancer (BC) is most commonly diagnosed worldwide. Liquiritigenin is a flavonoid found in various species of the Glycyrrhiza genus, showing anti–tumor activity. This article was to explore the influences of liquiritigenin on the biological behaviors of BC cells and its underlying mechanism. BC cells were treated with liquiritigenin alone or transfected with oe-HSP90 before liquiritigenin treatment. RT-qPCR and Western blotting were employed to examine the levels of HSP90, Snail, E-cadherin, HSC70, and LAMP-2A. Cell viability, proliferation, migration, and invasion were evaluated by performing MTT, colony formation, scratch, and Transwell assays, respectively. Liquiritigenin treatment reduced HSP90 and Snail levels and enhanced E-cadherin expression as well as inhibiting the proliferation, migration, and invasion of BC cells. Moreover, liquiritigenin treatment decreased the expression of HSC70 and LAMP-2A, proteins related to chaperone-mediated autophagy (CMA). HSP90 overexpression promoted the CMA, invasion, and migration of BC cells under liquiritigenin treatment. Liquiritigenin inhibits HSP90-mediated CMA, thereby suppressing BC cell growth.
Keywords: Breast neoplasms, Chaperone-mediated autophagy, Heat shock protein, Neoplasm metastasis, Neoplasms invasiveness, Rhizoma polygonati
View Full Text | Article as PDF |
Abstract | Figure & Table |
Pubmed | PMC |
Print this Page | Export to Citation |
ⓒ 2019. The Korean Journal of Physiology & Pharmacology. Powered by INFOrang Co., Ltd