Gastroprotective effect of cirsilineol against hydrochloric acid/ethanol-induced gastric ulcer in rats
Guojin Gong1,#, Rigetu Zhao2,#, Yuhui Zhu3, Jinfeng Yu4, Bin Wei5, Yan Xu6, Zhaoxun Cui7, and Guoying Liang8,*
1Department of Gastrointestinal Surgery, Xichang People's Hospital, Xichang 615000, 2Department of Gastroenterology, The Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao 028000, 3Department of Pharmacy, The Second Hospital of Jilin University, Changchun 130041, 4General Medicine Yantaishan Hospital, Yantai 264001, 5The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, 6Chongqing University Affiliated Tumor Hospital/Chongqing Cancer Institute, Chongqing 400030, 7Department of Oncology, Shaanxi Forest Industry Hospital, Xi’an 710000, 8Department of Liver, Spleen and Stomach Diseases, First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Heilongjiang 150040, China
This study was designed to evaluate the gastroprotective activity of cirsilineol in hydrochloric acid (HCl)/ethanol-induced gastric ulcer model. Cirsilineol was administered at the doses of 20 and 40 mg/kg in HCl/ethanol-induced rats. The gastroprotective ability was verified by determining the ulcer score, total acidity, hemoglobin, inflammatory cytokines, lipid peroxides, and enzymatic antioxidants superoxide dismutase (SOD) and catalase (CAT) in gastric tissue and serum biochemical analysis. The results showed a favorable increase in the hemoglobin level, antioxidant enzymes (SOD and CAT), restored electrochemical balance (carbon dioxide & anion gap) while a noticeable decrease in ulcer index, total acidity, lipid peroxides, inflammatory cytokines (interleukin-1 beta [IL-1β], IL-6, and tumor necrosis factor alpha) in rats treated with the cirsilineol. The serum biochemical analysis on liver markers (alkaline phosphatases, alanine aminotransferase, and aspartate aminotransferase), kidney markers (urea, creatinine, albumin, globulin, total protein), and lipid profile (triglyceride, high-density lipoprotein, total cholesterol) were attenuated by cirsilineol treatment in rats. Histopathology showed enhanced gastric protection and preserved the integrity of gastric mucosa upon cirsilineol administration. These results ultimately suggest that cirsilineol has gastroprotective effects that prevent the development of gastric ulcer.