Indexed in SCIE, Scopus, PubMed & PMC
pISSN 1226-4512 eISSN 2093-3827


home Article View

Original Article

Korean J Physiol Pharmacol 2021; 25(4): 297-305

Published online July 1, 2021

Copyright © Korean J Physiol Pharmacol.

Luteolin inhibits H2O2-induced cellular senescence via modulation of SIRT1 and p53

Ri Zhe Zhu#, Bing Si Li#, Shang Shang Gao, Jae Ho Seo*, and Byung-Min Choi*

Department of Biochemistry, Wonkwang University School of Medicine, Iksan 54538, Korea

Correspondence to:Jae Ho Seo
Byung-Min Choi

#These authors contributed equally to this work and should be considered as co-first authors.

Received: September 7, 2020; Revised: February 24, 2021; Accepted: April 5, 2021


Luteolin, a sort of flavonoid, has been reported to be involved in neuroprotective function via suppression of neuroinflammation. In this study, we investigated the protective effect of luteolin against oxidative stress-induced cellular senescence and its molecular mechanism using hydrogen peroxide (H2O2)-induced cellular senescence model in House Ear Institute-Organ of Corti 1 cells (HEI-OC1). Our results showed that luteolin attenuated senescent phenotypes including alterations of morphology, cell proliferation, senescence-associated β-galactosidase expression, DNA damage, as well as related molecules expression such as p53 and p21 in the oxidant challenged model. Interestingly, we found that luteolin induces expression of sirtuin 1 in dose- and time-dependent manners and it has protective role against H2O2-induced cellular senescence by upregulation of sirtuin 1 (SIRT1). In contrast, the inhibitory effect of luteolin on cellular senescence under oxidative stress was abolished by silencing of SIRT1. This study indicates that luteolin effectively protects against oxidative stress-induced cellular senescence through p53 and SIRT1. These results suggest that luteolin possesses therapeutic potentials against age-related hearing loss that are induced by oxidative stress.

Keywords: Cellular senescence, Hydrogen peroxide, Luteolin, Sirtuin 1, Tumor suppressor protein p53