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Original Article

Korean J Physiol Pharmacol 2010; 14(5): 279-283

Published online October 31, 2010 https://doi.org/10.4196/kjpp.2010.14.5.279

Copyright © Korean J Physiol Pharmacol.

Bark Constituents from Mushroom-detoxified Rhus verniciflua Suppress Kainic Acid-induced Neuronal Cell Death in Mouse Hippocampus

Jong-Seon Byun1, Yoon Hee Han1,4, Sung-Jun Hong1, Sung-Mi Hwang1, Yong-Soo Kwon2, Hee Jae Lee1, Sung-Soo Kim1, Myong-Jo Kim3,†, and Wanjoo Chun1,*

1Department of Pharmacology, College of Medicine, 2College of Pharmacy, 3Division of Bio-resources Technology, Kangwon National University, Chunchon 200-701, 4Department of Radiology, Ilsan Paik Hospital, Inje University, School of Medicine, Goyang 411-706, Korea

Abstract

Urushinol, a plant allergen, has significantly restricted the medical application of Rhus verniciflua, although it has been reported to possess a wide variety of biological activities such as anti-inflammatory, antioxidant, and anti-cancer actions. To reduce the urushinol content while maintaining the beneficial biological activities, mushroom-mediated fermentation of Rhus verniciflua was carried out and this method resulted in significantly attenuated allergenicity [1]. In the present study, to examine the neuroprotective properties of mushroom-fermented stem bark of Rhus verniciflua, two constituents were isolated from mushroom-fermented bark and their neuroprotective properties were examined in a mouse model of kainic acid (KA)-induced excitotoxicity. KA resulted in significant apoptotic neuronal cell death in the CA3 region of mouse hippocampus. However, seven daily administrations of RVH-1 or RVH-2 prior to KA injection significantly attenuated KA-induced pyramidal neuronal cell death in the CA3 region. Furthermore, pretreatment with RVH-1 and RVH-2 also suppressed KA-induced microglial activation in the mouse hippocampus. The present study demonstrates that RVH-1 and RVH-2 isolated from Rhus verniciflua and detoxified using mushroom species possess neuroprotective properties against KA-induced excitotoxicity. This leads to the possibility that detoxified Rhus verniciflua can be a valuable asset in herbal medicine.

Keywords: Kainic acid, Neuroprotection, Stigma-4-en-3-one, Stigma-4-en-3,6-dione