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Original Article

Korean J Physiol Pharmacol 2009; 13(6): 431-436

Published online December 30, 2009 https://doi.org/10.4196/kjpp.2009.13.6.431

Copyright © Korean J Physiol Pharmacol.

Overexpression of Ref-1 Inhibits Lead-induced Endothelial Cell Death via the Upregulation of Catalase

Kwon Ho Lee1,*, Sang Ki Lee1,*, Hyo Shin Kim1,2, Eun Jung Cho1,2, Hee Kyoung Joo1,2, Eun Ji Lee1,2, Ji Young Lee1,2, Myoung Soo Park1,2, Seok Jong Chang4, Chung-Hyun Cho1,2,3, Jin Bong Park1,2, and Byeong Hwa Jeon1,2,3

Departments of 1Physiology, 2Infection Signaling Network Research Center, and 3Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon 301-131, 4Department of Physiology, College of Medicine, Seonam University, Namwon 590-711, Korea

Abstract

The role of apurinic/apyrimidinic endonuclease1/redox factor-1 (Ref-1) on the lead (Pb)-induced cellular response was investigated in the cultured endothelial cells. Pb caused progressive cellular death in endothelial cells, which occurred in a concentration- and time-dependent manner. However, Ref-1 overexpression with AdRef-1 significantly inhibited Pb-induced cell death in the endothelial cells. Also the overexpression of Ref-1 significantly suppressed Pb-induced superoxide and hydrogen peroxide elevation in the endothelial cells. Pb exposure induced the downregulation of catalase, it was inhibited by the Ref-1 overexpression in the endothelial cells. Taken together, our data suggests that the overexpression of Ref-1 inhibited Pb-induced cell death via the upregulation of catalase in the cultured endothelial cells.

Keywords: Apurinic/apyrimidinic endonuclease1/redox factor-1 (Ref-1), Lead, Cell death, Endothelial cells, Catalase