Fig. 3. Analysis of action potentials (AP) in hiPSC-CMs.
(A) AP characteristics: A schematic showing the AP parameters measured in hiPSC-CMs, including maximum upstroke velocity (Vmax), amplitude (Amp), maximum diastolic potential (MDP), APD50, and APD80. (B) Representative AP: AP traces recorded from the CMC-006, CMC-011, CMC-016, and DPHC01i-AR cell lines using patch-clamps. Each trace displays the characteristic features of either ventricular-like or nodal-like APs. The DPHC01i-AR cell line exhibits prolonged APD with abnormal repolarization, reflective of its LQT background. (C) Proportions of AP types: The percentage distribution of ventricular-like, atrial-like, and nodal-like APs in each cell line (CMC-006, CMC-011, CMC-016, and DPHC01i-AR). A majority of cells in each cell line exhibit ventricular-like APs. hiPSC-CMs, human induced pluripotent stem cell-derived cardiomyocytes; LQT, long QT.
© Korean J Physiol Pharmacol
