Fig. 2. Immunocytochemical analysis of cardiomyocyte markers in hiPSC-CMs.
Immunofluorescence staining was performed to detect cardiomyocyte-specific proteins in the four hiPSC-CM lines (CMC-006, CMC-011, CMC-016, and DPHC01i-AR). Myosin heavy chain α (MHCα) is shown in red, cardiac Troponin T (cTnT) is shown in green, and nuclei are stained with 4’,6-diamidino-2-phenylindole (DAPI) in blue. The merged images display co-localization of these markers, confirming the expression of cardiomyocyte-specific proteins in all cell lines. The structural organization and marker expression exhibit slight variations across the cell lines, indicating successful differentiation with some heterogeneity in morphology. Scale bar: 100 μm. hiPSC-CMs, human induced pluripotent stem cell-derived cardiomyocytes.
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