Fig. 1. Gene expression analysis of cardiomyocyte-specific and ion channel genes in control and hiPSC-CM lines by RT-PCR.
(A) Cardiomyocyte-specific markers: The expression levels of TNNT2 and MYH6 were measured in control cells and four hiPSC-CM lines (CMC-006, CMC-011, CMC-016 derived from healthy individuals, and DPHC01i-AR derived from a patient with LQTS). The results are shown as fold changes normalized to the housekeeping gene GAPDH. The CMs of the four cell lines were normalized to their respective hiPS cells, with the hiPS cells set to 1 (upper panel). All hiPSC-CM cell lines exhibited significant upregulation of TNNT2 and MYH6 compared to the control, indicating successful differentiation into cardiomyocytes. (B) Ion channel gene expression: Key ion channel gene (SCN5A, KCNH2, KCND3, KCNQ1, and CACNA1C) expression levels were analyzed in the same cell lines. SCN5A, KCNH2, and KCNQ1 showed comparable expression levels across all cell lines, whereas KCND3 and CACNA1C exhibited variability in expression. Values are presented as mean ± SD. hiPSC-CM, human induced pluripotent stem cell-derived cardiomyocyte; cTnT, cardiac Troponin T; LQTS, long QT syndrome.
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