Fig. 3. Monotropein effect on vascular smooth muscle cell migration.
(A) Scratch experiment. Scale bar, 100 µm. n = 3 independent experiments. (B) Immunofluorescence co-staining of mouse aortic roots. The green color in the plaque represents MMP-2, red color represents Myh11. Scale bar, 50 µm. n = 3 independent experiments. (C) MMP-2 protein level in cells. n = 3 independent experiments. (D) Expression of MMP-2 in mouse aorta. n = 3 independent experiments. (E) Blank area statistics of scratch experiments. (F, G) Quantitative analysis of MMP-2 mRNA levels in cells. (H) Quantitative analysis of immunofluorescence staining in mouse aortic roots. (I) Quantitative analysis of MMP-2 protein levels in mouse aorta. Bar graphs depict mean ± SEM; one-way ANOVA *p < 0.05, **p < 0.01. MMP-2, matrix metalloproteinase-2; ox-LDL, oxidized low density lipoprotein; HFD, high-fat diet; MTP, monotropein; NCD, normal diet.
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