Fig. 5. LPS treatment increases galectin-3 expression in primary mouse microglial cells.
(A) Western blot analysis of galectin-3, Iba-1, and TREM-2 protein expressions in LPS (500 ng/ml)-treated microglial cells. β-actin was used as a loading control. (B) mRNA expression levels of il-6, il-1β, and tnf-α genes in LPS-treated primary mouse microglial cells using qRT-PCR. Data are presented as the mean ± SEM from five independent experiments. LPS, lipopolysaccharide; Iba-1, ionized calcium-binding adapter molecule-1; TREM-2, triggering receptor expressed on myeloid cells-2; il, interleukin; tnf-α, tumor necrosis factor-α. *p < 0.05 versus 0 h.
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