Fig. 4. Aging increases microglial galectin-3 in the mouse hippocampus.
(A) Western blot analysis and quantification of galectin-3 and TREM2 in the mouse hippocampus of 6-, 12-, and 24-month-old-mice. β-actin was used as a loading control. (B) Representative immunofluorescence images of the hippocampal region showing galectin-3 (green) and TREM-2 (red) staining. Nuclei were stained with DAPI. Scar bar = 50 μm. (C) Representative immunohistochemistry images of the hippocampal region showing galectin-3. The areas in black squares in the upper panel are magnified on the lower panel. Black and red arrows indicate vessel and microglial galectin-3, respectively. Quantification of galectin-3 in the hippocampus. Scar bar = 100 (insert, 30 μm). Data are shown as mean ± SEM. TREM-2, triggering receptor expressed on myeloid cells-2; DAPI, 4’,6-diamidino-2-phenylindole. *p < 0.05 versus 6M, †p < 0.05 versus 12M.
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