Fig. 2. Aging causes BBB-related proteins in the mouse hippocampus.
(A) Western blot analysis and quantification of ZO-1 in the mouse hippocampus of 6-, 12-, and 24-month-old-mice. β-actin was used as a loading control. (B) Representative immunofluorescence images of the hippocampal CA1 region showing AQP4 (green) and albumin (red). The white arrowheads indicate extravascular or extracellular albumins. The highly magnified images in the white boxes are shown in right panuls. Nuclei were stained with DAPI. (C) AQP4 fluoresence intensity in Fig. B. (D) Quantification of albumin in the hippocampus. The white arrow head indicates extracellular albumin. Scale bar = 50 μm (insert, 10 μm). Data are shown as mean ± SEM. BBB, blood-brain barrier; ZO-1, zonula occludens-1; AQP4, aquaporin-4; DAPI, 4’,6-diamidino-2-phenylindole. *p < 0.05 versus 6M, †p < 0.05 versus 12M.
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