Fig. 1. Aging increases neurodegeneration in the mouse hippocampus.
(A) Western blot analysis and quantification of pTau (Ser202)/Tau in the mouse hippocampus of 6-, 12-, and 24-month-old mice. β-actin was used as a loading control. (B) Representative images of the ProteoStat staining and TUNEL assay in the hippocampal CA1 region. The highly magnified images in the white boxes within the upper panels are shown in the bottom panels. The intensity of the ProteoStat dye is presented as fold changes. Scale bar = 50 µm (insert, 10 µm). (C) Cresyl violet-stained brain sections. The highly magnified images (CA1 and DG) in the white boxes are shown in the middle and lower panels. Quantification of the thickness of CA1 and DG. Scale bar = 100 µm (lower pannel, 20 µm). Data are shown as mean ± SEM. pTau, phosphorylated Tau; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; CA1, cornu ammonis1, DG, dentate gyrus. *p < 0.05 versus 6M, †p < 0.05 versus 12M.
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