Fig. 1. Effects of RSV on the cell viability and apoptosis in VSMCs.
(A) Cell viability measuring by MTT in VSMCs. The cells were treated with RSV (10, 40 and 100 μM) or digitonin (100 μg/ml) for 48 h. A digitonin was used as the positive control (PC). (B) Examination of apoptosis using Annexin V staining in VSMCs. The cells were treated with RSV (10 and 40 μM) or/and PDGF-BB (25 ng/ml) for 48 h. The percentage of indicated each cell population were determined and quantified. Data were analyzed using mean ± SEM. All experimental techniques are described in the Methods section. RSV, rosuvastatin; VSMCs, vascular smooth muscle cells; MTT, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; PDGF, platelet-derived growth factor. ***p < 0.001; ****p < 0.0001. n = 3 per group.
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