Fig. 1. Time-dependent increases in VSMC migration induced by HMGB1.
(A) A10 cells were stimulated with HMGB1 (100 ng/ml) for 0 to 48 h, and the cell migration was determined using a wound-healing assay. Photomicrographs of the cell monolayers at 0 to 48 h post-wounding are representative of 5 independent experiments. (B) The area of migrated cells in the wound area was calculated and expressed as % changes in the wound area. The area of migrated cells was quantified and expressed as the means ± SEM of 5 independent experiments. **p < 0.01 vs. corresponding value at 0 h, #p < 0.05 and ##p < 0.01 vs. value in the corresponding control. (C) The number of cells migrated into the wound area was counted at the indicated time. The data were quantified and expressed as the means ± SEM of 5 independent experiments. **p < 0.01 vs. corresponding value at 0 h, ##p < 0.01 vs. value in the corresponding control. VSMCs, vascular smooth muscle cells; HMGB1, high-mobility group box 1.
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