Fig. 4. Mebendazole (MBZ) disrupts microtubule organization and leads to tubulin polymerization.
(A, E) DMSO; (B, F) 0.1 μM MBZ; (C, G) 0.5 μM MBZ; (D, H) 1.0 μM MBZ. CML cells were treated with the indicated concentrations of MBZ for 24 h, and the alternation of microtubule organization and distribution was observed by fluorescence confocal microscopy. The scale bar represents 10 μm. (I) The protein level of α-tubulin in CML cells was detected by western blot. Tubulin polymerization assay was utilized to evaluate the polymerized (P) and soluble (S) α-tubulin in K562 cells (J) and K562/G01 cells (K) after incubated with MBZ, colchicine (Col), or paclitaxel (PTX) at indicated concentrations. The percentage of polymerized α-tubulin in K562 cells (L) and K562/G01 cells (M) was determined using the formula: %P = P/(P + S). The values are the mean ± SD of four independent experiments. CML, chronic myeloid leukemia; DMSO, dimethyl sulfoxide; ns, no significance. *p < 0.05 and **p < 0.01 vs. the controls.
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