Fig. 3. Mebendazole (MBZ) induces aberrant mitosis and multinucleation in CML cells.
(A, B) Cell cycle distribution was measured and analyzed using FCM. (C) The expression levels of cell cycle-associated proteins c-MYC, p21 and Cyclin B1 in CML cells treated with MBZ for 24 h were detected by western blot. (D, F) Morphological changes were observed by Liu’s staining after incubation with MBZ for 24 h (D) and 36 h (F). The scale bar represents 10 μm. (E, G) Mitotic index was determined by the proportion of mitotic cells after treatment with MBZ for 24 h and multi-nucleated cells were counted after 48 h (in a total of at least 100 cells in at least two random fields). The values are represented as the mean ± SD of three independent experiments. CML, chronic myeloid leukemia; FCM, flow cytometry; ns, no significance. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 vs. the controls.
© Korean J Physiol Pharmacol
