Fig. 2. Tasquinimod promoted apoptosis in OC/DDP cells by inhibiting HDAC4.
(A) Western blot analysis of the effect of Tasquinimod on HDAC4 expression. (B) CCK8 assays were used to evaluate the effect of Tasquinimod on cell viability. (C) Flow cytometry was used to detect apoptosis in SKOV3/DDP and A2780/DDP cells treated with Tasquinimod. (D, E) qRT-PCR and Western blot analyses were used to measure the expression levels of Bcl-2 and cleaved-Caspase-3 in tumor cells treated with Tasquinimod. All experiments were independently repeated three times. Tukey’s test and one-way ANOVA were used to analyze statistical significance. Values are presented as mean ± SD. OC, ovarian cancer; HDAC4, histone deacetylase 4; CCK8, cell counting kit-8; NC, negative control. *p < 0.05 vs. SKOV3/DDP + NC or A2780/DDP + NC group, #p < 0.05 vs. SKOV3/DDP or A2780/DDP group, &p < 0.05 vs. SKOV3/DDP + Tasquinimod or A2780/DDP + Tasquinimod group.
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