Fig. 6. Rescue analysis proved the association between the SHH-Gli1-AQP1 pathway and DR.
(A) Detection of DR related proteins STAT and VEGF by immunofluorescence stain. The magnifications are 200×. (B–D) Overexpression of Gli-1 and knockdown of SHH/AQP1 indicated the association between the SHH-Gli1-AQP1 pathway and DR. (E) Knockdown of AQP1 decreased the VEGF protein level compared with the model group, but over-expression of Gli-1 inhibited this effect (##p < 0.01 compared with the control group; *p < 0.05 compared with the model group, &p < 0.05 compared with the si-AQP1 group). n = 3 in each group, values are presented as mean ± SD. ##p < 0.01 compared with the control group; *p < 0.05 and **p < 0.01 compared with the model group, &p < 0.05 and &&p < 0.01 compared with the OE Gli-1 group. DR, diabetic retinopathy; APS, astragalus polysaccharide; SHH, Sonic Hedgehog.
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