Fig. 5. RT-qPCR and Western blotting analysis proved the association between the SHH-Gli1-AQP1 pathway and DR.
(A–C) mRNA levels detection of AQP1, Gli1, and SHH by RT-qPCR (n = 6). (D–G) Protein levels detection of AQP1, Gli1, and SHH by Western blotting (n = 3). ##p < 0.01 compared with the control group; *p < 0.05 and **p < 0.01 compared with the model group. Values are presented as mean ± SD. DR, diabetic retinopathy; APS, astragalus polysaccharide; SHH, Sonic Hedgehog.
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