Fig. 8. STAT phosphorylation by coexpression of PKD2 with TRPC.
(A) Effects of TRPC on STAT1 phosphorylation. HEK 293 cells were transfected with PKD2 and TRPC. Cell lysates were subjected to Western blot analysis using antibodies against total and phosphorylated STAT1. (B) Bar graphs showing mean levels of phosphorylated STAT1 relative to total STAT1 protein levels (n = 5). Statistical significance is denoted by an asterisk (*p < 0.05). The bar graphs represent the means ± S.E.M. (C) Effects of TRPC on STAT3 phosphorylation. HEK 293 cells were transfected with PKD2 and TRPC. Cell lysates were subjected to Western blot analysis using antibodies against total and phosphorylated STAT3. (D) Bar graphs showing mean levels of phosphorylated STAT3 relative to total STAT3 protein levels (n = 5). Statistical significance is denoted by an asterisk (**p < 0.01). (E) Effects of TRPC on STAT6 phosphorylation. STAT, signal transducer and activator of transcription; PKD, polycystic kidney disease; TRPC, classical transient receptor potential; n.s., not significant; IB, immunoblot.
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