Fig. 7. The effects of PKD2 on basal and La3+ induced TRPC5 currents.
(A) TRPC5 basal current without GTPγS. HEK 293 cells were co-transfected with TRPC5 and PKD2. The basal current of TRPC5 channels was increased by external cesium. The current amplitudes at −60 mV and +60 mV were plotted against time (the left panel) in HEK cells expressing PKD2 and TRPC5 (red triangle) or TRPC5 only (black triangle). The ramp pulses were applied every 10 sec. The I-V curves from HEK cells expressing PKD2 and TRPC5 (red line) or TRPC5 only (black line) showed a double-rectifying shape. (right panel) The bar graphs represent the means ± S.E.M of current density (pA/pF) at −60 mV. (B) TRPC5 activated with GTPγS. HEK 293 cells were co-transfected with TRPC5 and PKD2. The TRPC5 current was activated by GTPγS and external cesium. The current amplitudes at −60 mV and +60 mV were plotted against time (the left panel) in HEK cells expressing PKD2 and TRPC5 (red triangle) or TRPC5 only (black triangle). The ramp pulses were applied every 10 sec. The I-V curves from HEK cells expressing PKD2 and TRPC5 (red line) or TRPC5 only (black line) showed a double-rectifying shape. (right panel) The bar graphs represent the means ± S.E.M of current density (pA/pF) at −60 mV. (C) La3+ induced TRPC5 current. The TRPC5 current was activated by external La3+. The current amplitudes at −60 mV and +60 mV were plotted against time (the left panel) in HEK cells expressing PKD2 and TRPC5 (red triangle) or TRPC5 only (black triangle). The ramp pulses were applied every 10 sec. The I-V curves from HEK cells expressing PKD2 and TRPC5 (red line) or TRPC5 only (black line) showed a double-rectifying shape. (right panel) The bar graphs represent the means ± S.E.M of current density (pA/pF) at −60 mV. PKD, polycystic kidney disease; TRPC, classical transient receptor potential.
© Korean J Physiol Pharmacol
