Fig. 6. The effects of PKD2 on TRPC4 and TRPC5 channels stimulated by carbachol.
(A) HEK 293 cells were co-transfected with TRPC4 and PKD2, and M3 receptor was also expressed. TRPC4 current was activated by carbachol. Current amplitudes at −60 mV and +60 mV were plotted against time (left panel) in HEK cells expressing PKD2 and TRPC4 (red circles) or TRPC4 only (black circles). Ramp pulses were applied every 10 sec. I-V curves from HEK cells expressing PKD2 and TRPC4 (red line) or TRPC4 only (black line) showed a double-rectifying shape (right panel). Bar graphs represent the mean ± S.E.M of current density (pA/pF) at −60 mV. (B) HEK 293 cells were co-transfected with TRPC5 and PKD2, and M3 receptor was also expressed. TRPC5 current was activated by carbachol. Current amplitudes at −60 mV and +60 mV were plotted against time (left panel) in HEK cells expressing PKD2 and TRPC5 (red circles) or TRPC5 only (black circles). Ramp pulses were applied every 10 sec. I-V curves from HEK cells expressing PKD2 and TRPC5 (red line) or TRPC5 only (black line) showed a double-rectifying shape (right panel). Bar graphs represent the mean ± S.E.M of current density (pA/pF) at −60 mV. PKD, polycystic kidney disease; TRPC, classical transient receptor potential; CCh, carbachol.
© Korean J Physiol Pharmacol
