Fig. 5. The effects of PKD1 on TRPC5 channels.
(A) HEK 293 cells were co-transfected with TRPC5 and PKD(FL). The TRPC5 current was recorded without GTPγS, and the current amplitude was increased by 140 mmol/L Cs+. The current amplitudes at −60 mV and +60 mV were plotted against time (the left panel) in HEK cells expressing PKD1 and TRPC5 (red triangle) or TRPC5 only (black circle). The ramp pulses were applied every 10 sec. The I-V curves from HEK cells expressing PKD1 and TRPC5 (red line) or TRPC5 only (black line) showed a double-rectifying shape. (B) The bar graphs represent the means ± S.E.M of current density (pA/pF) at −60 mV in the absence of GTPγS infusion. (C) The current was recorded in TRPC5/PKD1 co-transfected HEK 293 cells infused with GTPγS. The I-V curves from HEK cells expressing PKD1 and TRPC5 (red line) or TRPC5 only (black line) showed a double-rectifying shape. (D) The bar graphs represent the means ± S.E.M of current density (pA/pF) at −60 mV in the presence of GTPγS infusion. (E) The effect of PKD1 on La3+-induced TRPC5 current. (F) The surface expression of TRPC5 in HEK cells coexpressing PKD1. Surface expression of TRPC5 was not increased by PKD1 as determined by co-expression and surface biotinylation. Immunoblots of surface and total were detected by anti-GFP antibody. Right panel: Bar graph showing the effect of PKD1 on the surface expression of TRPC5 (n = 3). *p < 0.05. PKD, polycystic kidney disease; TRPC, classical transient receptor potential, FL, full-length; CTF, C-terminal fragment; PM, plasma membrane; IB, immunoblot; n.s., not significant.
© Korean J Physiol Pharmacol
