Fig. 4. The interactions between TRPC and PKD2.
(A) HEK cells were transfected with TRPC1α, TRPC4β-Flag, or TRPC5-Flag along with PKD2-Myc and were used to test co-immunoprecipitation (Co-IP) of TRPC and PKD2. Anti-TRPC1 or anti-Flag antibody was used for immunoprecipitation (IP), and anti-PKD2 antibody was used for detecting PKD2-Myc. TRPC did not Co-IP with PKD2. (B, C) Anti-PKD2 antibody (H-280) was used for IP. TRPC4 or TRPC5 was detected with anti-Flag antibody (B), and TRPC1 was detected with anti-TRPC1 antibody (C). TRPC and PKD2 were not co-immunoprecipitated reciprocally. (D) FRET between TRPC and PKD2. FRET-detectable interactions occur between EYFP-PKD2 and ECFP-PKD2. Representative FRET images of ECFP-PKD2 co-expressed with EYFP-PKD2, EYFP-TRPC4, and TRPC4-EYFP are shown in the upper panel. In the lower panel, FRET images of ECFP-PKD2 co-expressed with EYFP-TRPC5 and a bar graph of FRET efficiency between PKD2 and TRPC are shown. TRPC, classical transient receptor potential; PKD, polycystic kidney disease; FRET, fluorescence resonance energy transfer; IB, immunoblot.
© Korean J Physiol Pharmacol
