Fig. 3. The effects of TRPC on the surface expression of PKD2.
(A) The surface expression of PKD2 in HEK cells coexpressing TRPC. Surface expression of PKD2-myc was increased by TRPC5 as determined by co-expression and surface biotinylation. Immunoblots of surface and total were detected by anti-PKD2 antibody (H-280). (B) Bar graphs showing mean levels of surface PKD2 relative to total PKD2 protein levels (black bar, n = 5), and mean levels of surface TRPC relative to total TRPC protein levels (white bar, n = 5). Statistical significance is denoted by an asterisk (*p < 0.05). (C) The surface expression of TRPC in HEK cells coexpressing PKD2. Surface expression of TRPC5 was increased by PKD2 as determined by co-expression and surface biotinylation. Immunoblots of surface and total were detected by anti-GFP antibody. (D) The schematic illustration of factors affecting the trafficking of PKD2 between the endoplasmic reticulum and the plasma membrane (PM). TRPC5 increases the surface expression of PKD2. TRPC, classical transient receptor potential; PKD, polycystic kidney disease; IB, immunoblot.
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