Fig. 1. The effects of PKD1 and GSK3
β on PKD2 expression. (A) Schematic illustration of factors affecting the trafficking of PKD2 between the endoplasmic reticulum (ER) and the plasma membrane (PM). (B, C) Surface expression of PKD2-GFP in HEK cells coexpressing PKD1 (B) or GSK3β (C). The surface expression of PKD2 was increased by PKD1 or GSK3β, as determined by co-expression and surface biotinylation. (D) Deletion of the PKD2 ER retention signal (PKD2 R740X) increased the surface expression of PKD2. Surface and total PKD2 were detected by immunoblotting with an anti-GFP antibody. PKD, polycystic kidney disease; IB, immunoblot.
© Korean J Physiol Pharmacol
