Fig. 4. Effect of p66shc KO on autophagy in the sciatic nerve of STZ-induced diabetes mice.
(A) Expression of autophagy-related proteins (p62, and LC3-II) by Western blotting in sciatic nerves from CON, T1DM, p66shc KO, and p66shc KO + T1DM mice. β-actin was used as the loading control. (B) Immunofluorescence of the expression of the autophagy-related proteins p62 (red), or LC3 (red), and p66shc (green) in sciatic nerves from CON, T1DM, p66shc KO, and p66shc KO + T1DM mice. (C) The fluorescent area of p62 and LC3 was quantified. Nuclei were stained with DAPI. Scale bar, 20 μm. Data represent means ± SEM, n = 15. CON, control (saline); T1DM, type 1 diabetes mice; p66shc KO, p66shc knockout mice; p66shc KO + T1DM, p66shc KO type 1 diabetic mice; DAPI, 4,6-diamidino-2-phenylindole. *p < 0.05 and ***p < 0.001, T1DM compared with CON; #p < 0.05, ##p < 0.01, and ###p < 0.001, p66shc KO + T1DM compared with T1DM.
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