Fig. 2. Downregulation of p66shc enhanced the expression of autophagy-related protein in high glucose condition.
Schwann cells transfected with siCON and sip66shc and treated with glucose (25 or 150 mM). (A) Expression of autophagy-related proteins (p62 and LC3-II) and p66shc by Western blotting in S16 rat Schwann cells. (B) Immunofluorescence images showing expression of p62 (red) or LC3 (red), and p66shc (green). (C) p62 and LC3 intensity was quantified per cell. Nuclei were stained with DAPI. Scale bar, 20 μm. β-actin was used as the loading control. Data represent means ± SEM, n = 3. DAPI, 4,6-diamidino-2-phenylindole. **p < 0.01 and ***p < 0.001, siCON + glucose 150 mM compared with siCON + glucose 25 mM; #p < 0.05, ##p < 0.01, and ###p < 0.001, sip66shc + glucose 150 mM compared with siCON + glucose 150 mM.
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