Fig. 1. p66shc-deficient Schwann cells attenuated oxidative stress, and early apoptosis triggered by high glucose concentrations.
S16 rat Schwann cells were treated with glucose for 72 h. (A) Percentages of apoptotic S16 rat Schwann cells by flow cytometry. (B) p66shc protein expression was measured by Western blotting. (C) Schwann cells transfected with siCON and sip66shc and treated with glucose (25 or 150 mM). ROS was measured by DCF-DA fluorescence and flow cytometry. (D) Percentages of apoptotic S16 rat Schwann cells by flow cytometry. β-actin was used as the loading control. Data represent means ± SEM, n = 3. ROS, reactive oxygen species; PI, propidium iodide; DCF-DA, 2,7- dichlorofluorescein. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, siCON + glucose 150 mM compared with siCON + glucose 25 mM; #p < 0.05, sip66shc + glucose 150 mM compared with siCON + glucose 150 mM.
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