Fig. 4. Regulation of migration by Orai1 and Orai3 in Caki-1 and Caki-2.
(A, B) Representative photos of wound-healing assays show that either 10% fetal bovine serum (FBS) or IGF-1 (100 nM) increases the migration of Caki-1 and Caki-2, respectively. (C, D) Representative images and the number of migrated cells demonstrating the impact of Orai1-3 silencing on 10% FBS-induced cell migration in Caki-1 and Caki-2, respectively. (E) Colony formation assay showing the impact of Orai1 or Orai3 knockdown on the proliferative capability of Caki-1 and Caki-2. Data are expressed as mean ± S.E.M. and analyzed with one-way ANOVA (A–D). **p ≤ 0.01, ***p ≤ 0.001. All experiments were performed three times with similar results.
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