Fig. 4. Histological analysis of tissue sections from mouse femurs after fine particulate matter (FPM) exposure using haematoxylin and eosin (H&E) and tartrate-resistant acid phosphatase (TRAP) staining.
Histological analysis was performed on femoral sections of mice on Day 19 after treatment (control group, n = 5, FPM-treated group, n = 7, and LPS-treated group, n = 3). (A) Representative images of H&E and TRAP staining from each group (40× and 100× magnification, Scale bars: 500 and 200 μm, respectively). (B) The trabecular area was measured using the ImageJ software. (C) The number of osteoclasts was counted using the ImageJ software. Bars represent mean ± standard deviation. LPS, lipopolysaccharide. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control.
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