Fig. 1. Experimental design of fine particulate matter (FPM)-induced effects in a mouse model.
(A) Strategic abstract of the experimental processes. Mice were divided into three independent groups (control group, n = 5, FPM-treated group, n = 7, and lipopolysaccharide [LPS]-treated group, n = 3). FPM (7.5 mg/kg) was administered every 3 days to ICR mice intraperitoneally for 12 or 19 days. The control group received phosphate-buffered saline (PBS, 10 ml/kg) instead, and LPS (5 mg/kg) was used as a positive control. (B) Body weight during the experiment. No significant change in body weight was observed between the intra- group during the FPM administration period. (C) Schematic representation of the micro-computed tomography (CT) scanning procedures for the assessment of microstructure of the femur. Overview of volume-of-interest (VOI) selection for the femur. VOI for the trabecular bone at distal metaphysis is shown in the red panels. VOI for the cortical bone at mid-diaphysis is shown in the blue panel. Scale bar, 1 mm.
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