Fig. 4. SAL alleviates lung injury in septic mice by inhibiting ferroptosis.
(A) H&E staining was used to analyze the morphological changes of lung tissue in mice (x20 and x200). Yellow arrows point to alveoli, red arrows to interstitial tissue, and black arrows to inflammatory infiltrates. (B) Pulmonary edema was evaluated by analyzing the lung W/D ratio. (C) TUNEL staining to evaluate apoptosis (x200). (D) The levels of TNF-α, IL-1β, IL-6 and IL-10 in BALF were measured by ELISA. (E) ROS level was detected by immunofluorescence (x200). (F) The content of Fe2+, glutathione (GSH) and malondialdehyde (MDA) were detected by kit. N = 6. Values are presented as mean ± SD. SAL, salidroside; W/D ratio, wet weight/dry weight ratio; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; TNF-α, tumor necrosis factor-α; IL, interleukin; BALF, bronchoalveolar lavage fluid; ROS, reactive oxygen species; CLP, cecal ligation and puncture; Fer-1, ferrostatin-1. *p < 0.05, **p < 0.01, ***p < 0.001.
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