Fig. 6. 6’-SL inhibits LPS-induced MMP9 and MCP-1 expression in mouse endothelium.
The mice were pretreated with 100 mg/kg of 6’-SL for 2 h prior to 10 mg/kg LPS for 6 h. Representative images of en face immunofluorescence staining of (A, B) MMP9 and (D, E) MCP-1 levels in the aorta endothelium of ICR mice. The endothelial junction was visualized though VE-cadherin staining, and nuclei were stained with DAPI. The scale bar indicates 30 µm. (C, F) The graph shows MMP9 and MCP-1 staining intensities in the mice aorta. Data are expressed as the mean ± SEM (n = 3). 6’-SL, 6’-sialyllactose; LPS, lipopolysaccharide; MMP9, matrix metalloproteinase-9; MCP-1, monocyte chemoattractant protein-1; VE-cadherin, vascular endothelial-cadherin; DAPI, 4’,6-diamidino-2-phenylindole. ***p < 0.001 compared with the control group, ###p < 0.001 compared with the LPS-treated group.
© Korean J Physiol Pharmacol
