Fig. 3. Effect of 6-SL on LPS-induced ROS production.
(A, B) The DHE assay showed a remarkable increase in ROS production in the cells. Raw 264.7 cells were pre-treated with 25, 50, 100, and 200 µM of 6’-SL for 1 h, followed by treatment with 1 µg/ml LPS for 24 h. The quantified graph indicates DHE fluorescence using ImageJ. Data are expressed as the mean ± SEM (n = 4). The scale bar represents 100 µm. 6’-SL, 6’-sialyllactose; LPS, lipopolysaccharide; ROS, reactive oxygen species; DHE, dihydroethidium; DAPI, 4’,6-diamidino-2-phenylindole. ***p < 0.001 compared to the control group, #p < 0.05 compared to the LPS-treated group.
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