Fig. 1. Effect of 6’-SL on cell viability and Akt and p38 signaling pathway in RAW 264.7 cells.
To determine the optimal concentration of 6’-SL for our experiments, we assessed the impact on inflammation signaling in RAW 264.7 cells via Western blotting. (A) RAW 264.7 cells were pre-exposed to 25, 50, 100, and 200 µM of 6’-SL for 1 h, followed by treatment with 1 µg/ml LPS for 24 h. Cell viability was assessed using the MTT assay. The results are presented as mean ± SEM, with n = 5 in each group. (B) RAW 264.7 cells were pre-treated with varying concentrations of 6’-SL for 1 h, followed by 1 µg/ml LPS treatment for 1 h. Total cell lysates were subjected to Western blot analysis using specific antibodies. 6’-SL, 6’-sialyllactose; Akt, protein kinase B; LPS, lipopolysaccharide; MTT, 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay.
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