Fig. 5. Structural and docking analyses of anti-ATXN1 and anti-STAT3 nanobodies.
(A, B) Model structure of anti-ATXN1 (A, left) and anti-STAT3 nanobodies (B, left), with the CDR regions colored accordingly: pink for CDR1, yellow for CDR2, and purple for CDR3. The right panels of (A) and (B) show surface representations of the ATXN1 (PDB ID: 1OA8) and STAT3 (PDB ID: 6NJS) molecules within the docking models of the ATXN1/anti-ATXN1 and STAT3/anti-STAT3 nanobody interactions. Expected epitope regions are color-coded based on their model-specific or shared presence in the first, second, and third models, which enhances our understanding of the binding interfaces. The epitope regions of the first, second, third models are colored pink, green, and purple, respectively. The epitope regions shared by the first and second models are colored yellow. The epitope regions shared by the second and third models are colored blue. The epitope regions shared by the first and third models are red teal. The nanobody structures of the first, second, third models are colored pink, green, and purple, respectively. ATXN1, ataxin 1; STAT3, signal transducer and activator of transcription 3; CDR, complementarity-determining region.
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