Fig. 3. Construction of the synthetic nanobody library.
(A) Quantification of the amino acid composition in the CDR3 region of the synthetic nanobody library using color coding for different amino acid types: sky blue for glycine, purple for histidine, light green for hydrophobic (aliphatic) amino acids, green for aromatic amino acids, yellow for polar amino acids, red for negatively charged amino acids, and blue for positively charged amino acids. (B) Outlines the cloning strategy used to construct the nanobody library, visualized using BioRender.com. (C) The size of 10 different nanobody sub-libraries. (D) Evaluation of the quality of these 10 nanobody sub-libraries based on sequencing results.
© Korean J Physiol Pharmacol
