Fig. 5. Role of PDGFR-
β signaling on monocyte chemoattractant protein 1 (MCP-1) expression in vascular smooth muscle cells (VSMCs) stressed with mechanical stretch (MS). The cultured VSMCs were transfected with PDGFR-β siRNA (200 nM) for 24 h, and then stimulated by 10% MS for 6 h. The expression levels of PDGFR-β and MCP-1 were determined by Western blotting using β-actin as an internal control. The blots are representative of 4 independent experiments (A). Relative intensities of PDGFR-β (B) and MCP-1 (C) to β-actin were quantified and expressed as the means ± SEM of 4 independent experiments. **p < 0.01 vs. control. ##p < 0.01 vs. corresponding value in negative control. (D) MCP-1 expression in 10% MS-stimulated cells was compared with that in control cells, and the relative increase rate was quantified. Data were expressed as the means ± SEM of 4 independent experiments. **p < 0.01 vs. negative control.
© Korean J Physiol Pharmacol
