Fig. 4. Individual roles of the PDGFR isoforms on high mobility group box 1 (HMGB1) release in mechanical stretch (MS)-stimulated vascular smooth muscle cells (VSMCs).
The cultured VSMCs were transfected with siRNAs (200 nM) for PDGFR-α (A) or PDGFR-β (B) for 24 h, and then stimulated with 10% MS for 6 h. The expression levels of PDGFR-α and PDGFR-β isoforms were determined by Western blotting using β-actin as an internal control. Relative intensities were quantified and expressed as the means ± SEM of 4 independent experiments. **p < 0.01 vs. control. ##p < 0.01 vs. corresponding value in negative control. (C, D) VSMCs transfected with PDGFR-α or PDGFR- β siRNA (200 nM) for 24 h were stimulated with 10% MS for 6 h. The HMGB1 released in the culture media were quantified by ELISA. The concentration of HMGB1 were quantified and expressed as the means ± SEM of 4 independent experiments. **p < 0.01 vs. control. ##p < 0.01 vs. corresponding value in negative control.
© Korean J Physiol Pharmacol
