Fig. 2. High mobility group box 1 (HMGB1) release and its role on monocyte chemoattractant protein 1 (MCP-1) expression in vascular smooth muscle cells (VSMCs) stimulated with mechanical stretch (MS).
(A) Force- and time-dependent effects of MS on HMGB1 release in VSMCs. Rat VSMCs were stimulated with 3% and 10% MS for 0–12 h, and then HMGB1 released into cell culture media was measured by ELISA. The concentration of HMGB1 released into culture media was quantified and expressed as the means ± SEM of 4 independent experiments **p < 0.01 vs. corresponding value in the 3% MS group. (B) Role of HMGB1 on MCP-1 expression in VSMCs stimulated with MS. Rat VSMCs were pretreated with an inhibitor for HMGB1 (glycyrrhizin, 0–10 nM) for 24 h, and the cells were then stimulated with 10% MS for 12 h. The expression of the MCP-1 protein was determined by Western blot. β-Actin was used as an internal control. The blots are representative of 4 independent experiments. Relative intensities to β-actin were quantified and expressed as the means ± SEM of 4 independent experiments. **p < 0.01 vs. control. ##p < 0.01 vs. vehicle.
© Korean J Physiol Pharmacol
