Fig. 1. Force- and time-dependent effects of mechanical stretch (MS) on monocyte chemoattractant protein 1 (MCP-1) expression in vascular smooth muscle cells (VSMCs).
(A) Rat aortic VSMCs were stressed with MS (0, 3, 5 and 10% strain, 60 cycles/min) using Flexcell Tension Plus FX-4000T system for 12 h. MCP-1 expression in VSMCs was measured by Western blot, and the blots are representative of 5 independent experiments. β-Actin was used as an internal control. Quantitative results were expressed as the mean ± SEM of 5 independent experiments. **p < 0.01 vs. control. (B) The cultured VSMCs were stressed with 10% MS for 12 h, and MCP-1 expression in VSMCs was measured by Western blotting. β-Actin was used as an internal control. The blots are representative of 4 independent experiments. Quantitative results were expressed as the means ± SEM of 4 independent experiments. *p < 0.05; **p < 0.01 vs. control.
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