Fig. 1. mGluR5 antagonist inhibits the spike firing of low [Mg²⁺]_o-induced interictal epileptiform activity in the CA1 region of rat hippocampal slice. Exposure to Mg²⁺-free ACSF induced interictal epileptiform activity in the CA1 (A1) and CA3 (B1) regions of rat hippocampal slices. The mGluR5 antagonist MPEP (50 μM) inhibited the spike firing of the low [Mg²⁺]_o-induced interictal epileptiform activity, but the mGluR1 antagonist LY367385 (100 μM) did not affect the interictal epileptiform activity in the CA1 (A1). Graph summarizes the spike firing and amplitude of the low [Mg²⁺]_o- induced epileptiform activity in non (control, n = 7)-, MPEP (n = 6)- and LY367385 (n = 6)-treated groups in the CA1 (A2) and in non (control, n = 6)-, MPEP (n = 7)- and LY367385 (n = 9)-treated groups CA3 (B2) region. Data are expressed as means ± SEM. mGluR, metabotropic glutamate receptor; [Mg²⁺]_o, extracellular Mg²⁺ concentration; ACSF, artificial cerebrospinal fluid; MPEP, 6-Methyl-2-(phenylethynyl) pyridine. *p < 0.05 relative to respective control (one way ANOVA with Bonferroni’s test).

Korean J Physiol Pharmacol 2024;28:413-422 https://doi.org/10.4196/kjpp.2024.28.5.413

© Korean J Physiol Pharmacol