Fig. 4. Liquiritigenin inhibits HSP90-mediated CMA to suppress BC cell invasion and migration.
RT-qPCR (A, C) and Western blotting (B, D) were performed to detect the expression of CMA-related HSC70 and LAMP-2A; (E) MTT assay to detect cell viability; (F) colony formation assay to detect cell proliferation; (G) scratch assay to detect cell migration; (H) Transwell assay to detect cell invasion; and (I) Western blotting to detect the expression of invasion and migration-related proteins Snail and E-cadherin. The data are expressed as mean ± standard deviation. Each cell experiment was repeated thrice. HSP90, heat shock protein 90; CMA, chaperone-mediated autophagy; BC, breast cancer; RT-qPCR, reverse transcription quantitative polymerase chain reaction; LAMP-2A, lysosome-associated membrane protein type 2A. #p < 0.05, compared to oe-NC group.
© Korean J Physiol Pharmacol
