Fig. 5. Reduction of mitochondrial superoxide by diphenyleneiodonium (DPI).
(A) Plots of averaged Mito-SOX fluorescence, normalized to the levels (F0) just prior to the application of DPI (3 μM, n = 4; 30 μM, n = 5; 100 μM, n = 4), versus recording time, showing decrease of mitochondrial superoxide levels by DPI in a concentration-dependent manner. (B) Summary of Mito-SOX fluorescence ratio measured at 100-s after the onset of DPI applications, showing DPI-induced signal reductions at 3 μM, 30 μM, and 100 μM DPI. **p < 0.01, *p < 0.05 vs. control (Con) (paired t-test).
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