Fig. 10. 3,3′,4,4′-tetrachlorobiphenyl (PCB77) inhibits lipopolysaccharide-induced production of tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in mouse macrophages.
(A) Raw264.7 cells were treated with PCB77 (10 μM) for 12 or 24 h. The release rates of TNF-α and IL-10 into the culture supernatant were measured using an enzyme-linked immunosorbent assay (ELISA). (B) Raw264.7 cells were pretreated with PCB77 for 24 h and then stimulated with lipopolysaccharide (LPS) for 12 or 24 h. The release rates of TNF-α and IL-10 into the culture supernatant were measured using an ELISA. All experiments were performed in triplicate and compared with a one-way analysis of variance followed by Tukey’s post-hoc test for multiple comparisons; **p < 0.01; ***p < 0.001, and not significant (ns) (p > 0.05).
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