Fig. 4. POLR1D activated by YY1 can regulate CRC cell proliferation and angiogenesis ability through p38 MAPK signal pathway.
After SW480 and HT-29 cells were transfected with si-NC, si-YY1, pcDNA3.1 and POLR1D or 10 μM SB 203580, the cell proliferation was detected by using CCK-8 assay (A) and clone formation assay (B). The angiogenesis of HUVECs was assessed by tube formation assay. Scale bar, 20 μm (C). Western blot was used to detect the protein expression levels of p38 MAPK signal pathway related proteins (D). Data were presented as mean ± SD. CRC, colorectal cancer; HUVECs, human umbilical vein endothelial cells. *p < 0.05, **p < 0.01, ***p < 0.001.
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