Fig. 3. YY1 can bind with the promoter of POLR1D to increase its transcription and elevate its expression level.
SW480 and HT-29 cells were transfected with pcDNA3.1, YY1, si-NC or si-YY1. JASPAR was used to predict the binding sites and the mutant sequences between YY1 and POLR1D promoter (A). The interaction between YY1 and POLR1D was verified by dual luciferase reporter gene assay (B) and ChIP (C). qRT-PCR (D) and Western blot (E) were used to detect POLR1D mRNA and protein expression levels. Data were presented as mean ± SD. CRC, colorectal cancer. *p < 0.05, **p < 0.01.
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